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Temporal tracking of microglia activation in neurodegeneration at single-cell resolution

GSE103334 Mus musculus Expression profiling by high throughput sequencing 2208 samples Submitted 2017/10/10 Platform GPL13112
Summary
In this study, we used single-cell RNA-sequencing to gain unprecedented insight into the phenotypic heterogeneity and the transcriptional dynamics of microglia cells during the progression of neurodegeneration. Briefly, by using a severe neurodegeneration mouse model with Alzheimer’s-like pathology and phenotypes (CK-p25 model), we surveyed microglia activation by RNA sequencing longitudinally at fine temporal- and single-cell resolution. In summary, our work identified previously unobserved heterogeneity in the response of microglia to neurodegeneration, discovered novel microglia cell states, revealed the trajectory of cellular reprogramming of microglia in response to neurodegeneration, and uncovered the underlying transcriptional programs. These insights into the molecular programs underlying microglia activation provided by our study may pave the way for designing new rational and efficient strategies to treat Alzheimer’s and other neurodegenerative diseases.
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Direct links to NCBI, no account and no request form: the whole study as GSE103334_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 2208 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA401847 and SRA study SRP124513. Searching any of these in the dataset finder brings you back here.

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