← BioTransfer GEO Dataset Finder
GEO series

Glutamine deprivation enhances metabolic reprogramming during kidney organoid directed differentiation

GSE126719 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/03/20 Platform GPL11154
Summary
In this study, we systematically performed comprehensive metabolite and transcriptome profiling of human pluripotent stem cells (hPSCs) during differentiation into NPCs and later into multicellular organoids. We found activation of distinct metabolic pathways during early stage progression from hPSCs to NPCs; however, NPCs, organoids, and the intervening developmental stages between them shared similar metabolic profiles across this later stage of maturation. Importantly, the Alanine-aspartate-glutamate and glutamine pathways were significantly altered during the first stages of differentiation. Moreover, hPSCs survived glutamine deprivation during in vitro differentiation, and NPCs were successfully generated both in the absence and presence of glutamine and glutamate. Surprisingly, metabolic deprivation resulted in enhanced maturation, specifically in podocytes, as evidenced through single cell RNA-Seq analysis. These findings highlight the critical regulatory role of glutamine metabolism in the derivation of nephron progenitor cells and kidney organoids.
Published in
Parallel multiOMIC analysis reveals glutamine deprivation enhances directed differentiation of renal organoids
Sarami I, Hekman KE, Gupta AK et al. · bioRxiv : the preprint server for biology 2025 · PMID 40060393 · doi:10.1101/2025.02.27.640060
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE126719_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA523040 and SRA study SRP186199. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.