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RNA-seq on in vitro 3D structure of Klf5-KO biliary epithelial cells

GSE145420 Mus musculus (mouse) RNA-seq (gene expression) 15 samples Submitted 2026/07/31 Platform GPL17021
At a glance

RNA-seq (gene expression) · mouse · 15 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
15 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
In Klf5 knockout mice, the ductular reaction upon cholestatic liver injury is severely suppressed. We developed an in vitro 3D branching system that mimics the ductular reaction. In this system, the biliary cells were detached from the branching structure when Klf5 was lost. In order to unravel the roles of Klf5 and identify its target molecules that function to prevent the cells from detaching, we conducted RNA-seq analysis on the biliary cells from 3D in vitro branching structure.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE145420_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA607198 and SRA study SRP249779. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 15 more — browse all 15 samples with per-sample file links →

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