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A Single-cell Transcriptomic Atlas of Human Intervertebral Disc

GSE160756 Homo sapiens Expression profiling by high throughput sequencing 7 samples Submitted 2021/08/20 Platform GPL20795
Summary
Using high-precision transcriptomics to systematically map the atlas of human intervertebral disc (IVD) at single-cell resolution. Consequently, we found remarkable cellular diversity in the human IVD and identified a set of signature markers to recognize the cell types spatially. Furthermore, we deciphered a biological classification of chondrocyte subclusters with distinct role in the ECM homeostasis.Notably, the critical clues were also discovered for progenitor cells with bi-lineage differentiation trajectories in the nucleus pulposus, which discriminatively marked by the ancestry molecules PDGFRA and PROCR and highly enriched PDGF network. Finally, we uncovered the potential vital factors maintaining the IVD homeostasis from the intercellular crosstalk based on the signaling network landscape of the IVD microenvironment.
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Direct links to NCBI, no account and no request form: the whole study as GSE160756_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA674356 and SRA study SRP291007. Searching any of these in the dataset finder brings you back here.

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