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Gene regulation using the flap endonuclease 1 guided by mis-hairpin DNA probes

GSE171834 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/04/11 Platform GPL24676
Summary
Here we characterized a genetic regulatory system named as the hpDNA-assisted structure-guided nuclease mediating interference (HpSGNi) system, which was composed of flap endonuclease 1 (FEN1) and mis-hairpin DNA probe (mis-hpDNA). To investigate gene regulation effective of the HpSGNi system, we co-transformed HEK293A (human embryonic kidney epithelial cells) with plasmids encoding FEN1 with NES and two groups of mis-hpDNAs targeting the EGFP gene and negative control, respectively. RNA-seq was used to examine the regulation effective. We proved the HpSGNi system can knock down specific gene in human cells. At the same time, it indicated that a degree of off-targets occur in HpSGNi-treated cells.
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Also filed as BioProject PRJNA721171 and SRA study SRP314340. Searching any of these in the dataset finder brings you back here.

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