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Impact of CTD truncation on co-transcriptional splicing.

GSE175663 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/05/27 Platform GPL24676
Summary
For overexpression of exogenous RPB1, 10 ug of purified HA-tagged RPB1 plasmids (wildtype or CTD-truncated, both α-amanitin resistant) were transfected into HeLa S3 cells per 10 cm dish using TurboFect transfection reagent (Thermo Fisher Scientific). Culture medium was changed to include 2 ug/ml of Doxycycline 12 hrs post-transfection for inducing exogenous gene expression and then treated with 2.5 ug/ml α-amanitin to block the function of endogenous RPB1. Cells were further cultured for 42 hrs before harvesting for subsequent analysis.
Published in
The Internalization of Neurotensin by the Low-Affinity Neurotensin Receptors (NTSR2 and vNTSR2) Activates ERK 1/2 in Glioma Cells and Allows Neurotensin-Polyplex Transfection of tGAS1
Ayala-Sarmiento AE, Martinez-Fong D, Segovia J · Cellular and molecular neurobiology 2015 · PMID 25772140 · doi:10.1007/s10571-015-0172-z
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Also filed as BioProject PRJNA733164 and SRA study SRP321637. Searching any of these in the dataset finder brings you back here.

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