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PTEN controls alternative splicing of autism spectrum disorder-associated transcripts in primary neurons

GSE182975 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/08/16 Platform GPL21103
Summary
Purpose: To identify alternative splicing events in primary Pten-/- neurons as a model for autism spectrum disorder (ASD). Methods: RNA isolation from DIV14 control and cre-mediated Pten-/- primary cortical neuronal, glia-depleted cultures. PE RNA-Seq using Illumina4000 system. Identification of alternatively spliced transcripts in Pten-/- cultures using rMATS and iREAD. Results: More than 900 alternative splicing events were identified in Pten-/- neurons. These are largely related to synaptic and gene expression regulatory processes. Importantly, more than 80 additional autism-associated risk genes were affected upon Pten loss. Conclusion: PTEN-ASD seems to display a multifactorial condition rather than a monogenetic disorder.
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Also filed as BioProject PRJNA758573 and SRA study SRP334560. Searching any of these in the dataset finder brings you back here.

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