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Single Nucleus Transcriptomics Identifies Separate Classes of UCP1 and Futile Cycle Adipocytes [human snRNA-seq]

GSE184687 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/07/30 Platform GPL24676
Summary
Adipose tissue can recruit catabolic adipocytes which utilize chemical energy to dissipate heat. This process occurs either by uncoupled respiration through uncoupling protein 1 (UCP1) or by utilizing ATP-dependent futile cycles (FCs). However, it remains unclear how these pathways coexist since both processes rely on the mitochondrial membrane potential. Utilizing single-nucleus RNA-sequencing to deconvolute the heterogeneity of subcutaneous adipose tissue in mice and humans, we identify at least 2 distinct subpopulations of beige adipocytes: FC-beige and UCP1-beige adipocytes. Importantly, we demonstrate that the FC-utilizing beige subpopulation is metabolically highly active and utilizes FCs to dissipate energy thus contributing to thermogenesis independent of UCP1. Furthermore, FC-beige adipocytes are important drivers of systemic energy homeostasis and linked to glucose metabolism and obesity resistance in humans. Taken together, our findings identify a noncanonical thermogenic adipocyte subpopulation, which could be an important regulator of energy homeostasis in mammals.
Published in
Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes
Wang T, Sharma AK, Wu C et al. · Cell metabolism 2024 · PMID 39084216 · doi:10.1016/j.cmet.2024.07.005
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Also filed as BioProject PRJNA765667 and SRA study SRP338445. Searching any of these in the dataset finder brings you back here.

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