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Time-resolved RNA sequencing reveals distinct post-transcriptional regimes during zebrafish embryogenesis

GSE185283 Danio rerio (zebrafish) RNA-seq (gene expression) 72 samples Submitted 2022/12/31 Platform GPL18413Platform GPL17021
At a glance

RNA-seq (gene expression) · zebrafish · 72 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Danio rerio (zebrafish)
Samples
72 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
SLAMseq resolves the kinetics of maternal and zygotic gene expression during early zebrafish embryogenesisCell reports · 2023
Summary (written by the submitters)
We report the characterization of transcript dynamics during maternal to zygotic transition in Zebrafish (MZT) using thiol-linked alkylation for the metabolic sequencing of RNA (SLAMseq).
Published in
SLAMseq resolves the kinetics of maternal and zygotic gene expression during early zebrafish embryogenesis
Bhat P, Cabrera-Quio LE, Herzog VA et al. · Cell reports 2023 · PMID 36757845 · doi:10.1016/j.celrep.2023.112070
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE185283_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA768450 and SRA study SRP339965. Searching any of these in the dataset finder brings you back here.

Samples in this study

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.