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Single cell transcriptome level assessment of human platelet-biased hematopoietic stem cells isolated from bone marrow samples obtained from healthy young and old adult donors.

GSE188889 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/23 Platform GPL24676
Summary
Functional heterogeneity of hematopoietic stem cells (HSCs) heterogeneity has been extensively described in mice, but so far not described in human bone marrow (BM). In particular, HSCs biased towards platelet formation have been identified, and shown to be expanded during ageing and implicated as cells-of-origin for essential thrombocythemia (ET). By combining xenografting of molecularly barcoded adult human BM HSCs and high-throughput single cell RNA sequencing we here identify human BM HSCs that are molecularly and functionally platelet-biased. Furthermore, a quantitative comparison of transcriptomes from young and aged BM HSCs showed that both the proportion of platelet-biased HSCs and their level of molecular platelet priming increases with age.
Published in
Hematopoietic stem cell heterogeneity and age-associated platelet bias are evolutionarily conserved
Aksöz M, Gafencu GA, Stoilova B et al. · Science immunology 2024 · PMID 39178276 · doi:10.1126/sciimmunol.adk3469
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Also filed as BioProject PRJNA780572. Searching any of these in the dataset finder brings you back here.

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