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DROSHA and DICER RNA products control BMI1-dependent transcriptional repression at DNA damage sites

GSE193821 Homo sapiens (human) RNA-seq (gene expression) 26 samples Submitted 2025/10/09 Platform GPL21697
At a glance

RNA-seq (gene expression) · human · 26 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
26 samples: 8 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand breakCell reports · 2025
Summary (written by the submitters)
The activation of the DNA-damage response (DDR) enforces the transcriptional silencing of genes near DNA double-strand breaks (DSBs), a process called DSB-induced silencing in cis (DISC). DISC involves the kinase ATM and the polycomb repressive complex 1 (PRC1) component BMI1. Conversely, DSBs also trigger transcription of damage-induced long non-coding RNAs (dilncRNAs) in an MRE11-RAD50-NBS1 (MRN)-complex-dependent manner.
Read the full summary ↓
MRN recruits the ribonuclease DROSHA, which, along with DICER, enhances DDR signaling and repair. We show that dilncRNAs, DROSHA, and DICER regulate DISC. MRN or ATM inhibition disrupts DISC, while enoxacin, a DICER activator, restores it even without ATM activity. Mechanistically, DROSHA and DICER enable BMI1 recruitment and H2A-K119 ubiquitination at DSBs. BMI1 interacts with DROSHA and dilncRNAs in a DICER-dependent manner. Blocking dilncRNAs by antisense oligonucleotide and Cas13 reduces BMI1 recruitment and DISC. We propose that DROSHA, DICER, and dilncRNAs mediate DISC by promoting PRC1 recruitment and chromatin modification at DSBs.
Published in
DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break
Esposito F, Capozzo I, Riccardi A et al. · Cell reports 2025 · PMID 41348540 · doi:10.1016/j.celrep.2025.116605
This dataset
Open GSE193821 in finder → Find more human RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE193821_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA798043 and SRA study SRP355430. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41348540) ↗
Study design
8 conditions, mostly in triplicate
siDIC-DRO Induced ×5 siDIC-DRO Uninduced ×5 siATM Induced ×3 siATM Uninduced ×3 siCTL Induced ×3 siCTL Uninduced ×3 siCTL induced ×2 siCTL uninduced ×2

Supports a between-group comparison across 26 samples.

How we read this: 8 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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