← BioTransfer GEO Dataset Finder
GEO series

Identification of an RNA-binding region in 18S dimethyladenosine methyltransferase DIMT1 essential for proliferation of acute myeloid leukemia cells

GSE197276 Homo sapiens Expression profiling by high throughput sequencing; Other 8 samples Submitted 2024/04/09 Platform GPL21697
Summary
Aberrant assembly and function of ribosomes are implicated in cancers, including acute myeloid leukemia (AML). Here, we find that 18S rRNA methyltransferase DIMT1 is essential for AML proliferation through a non-catalytic function. We demonstrate that a positively charged cleft on DIMT1 plays an important role in RNA binding. Mutations at the identified cleft (5mutA-DIMT1) significantly weakens binding of DIMT1 to rRNA in vitro and in cells. Strikingly, the RNA-binding deficient 5mutA-DIMT1 is predominantly located in the nucleoplasm, in contrast with the primarily nucleolar localization of wild-type DIMT1. Furthermore, we demonstrate that rRNA binding is required for DIMT1 to undergo liquid-liquid phase separation in vitro, which likely promotes DIMT1’s nucleolar localization and its role in rRNA processing in cells. Importantly, re-expression of wild-type but not 5mutA-DIMT1 supports AML proliferation in competition-based cell proliferation assays. These results highlight the importance of targeting the catalytically independent RNA-binding activity of DIMT1 for therapy of AML.
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE197276_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA809655 and SRA study SRP361097. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.