H3K27me3 spreading organizes canonical PRC1 chromatin architecture to regulate developmental programs [scRNA-seq]
Single-cell RNA-seq · human · 11 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
- Organism
- Homo sapiens (human)
- Samples
- 11 samples: 3 × HSJ019, K27M-KO, vs 2 × DIPG13, K27M, vs 2 × DIPG13, K27M-KO, vs 2 × HSJ019, K27M,Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- H3K27me3 spreading organizes canonical PRC1 chromatin architecture to regulate developmental programsNature genetics · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE205248_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA844274 and SRA study SRP377947. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 9 samples.
How we read this: 4 replicated groups read from 11 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM6209145 BT245, K27M, replicate 1
- GSM6209146 BT245, K27M-KO, replicate 1
- GSM6209147 DIPG13, K27M, replicate 1 DIPG13, K27M,
- GSM6209148 DIPG13, K27M, replicate 2 DIPG13, K27M,
- GSM6209149 DIPG13, K27M-KO, replicate 1 DIPG13, K27M-KO,
- GSM6209150 DIPG13, K27M-KO, replicate 2 DIPG13, K27M-KO,
- GSM6209151 HSJ019, K27M, replicate 1 HSJ019, K27M,
- GSM6209152 HSJ019, K27M, replicate 2 HSJ019, K27M,
- GSM6209153 HSJ019, K27M-KO, replicate 1 HSJ019, K27M-KO,
- GSM6209154 HSJ019, K27M-KO, replicate 2 HSJ019, K27M-KO,
- GSM6209155 HSJ019, K27M-KO, replicate 3 HSJ019, K27M-KO,
- GSE312029 Spatiotemporal remodeling of chromatin topology architecture and H3K27me3 redistribution underlies vascular pathology in Hutchinson-Gilford progeria syndrome [CUT&Tag] 84 samples shares h3k27me3, architecture, chromatin
- GSE337246 Integrated single-cell profiling of RNA and DNA interactomes reveals targetable chromatin architectures in cancer [scRNA-Seq organoids] 35 samples shares architecture, chromatin, scrna-seq
- GSE293771 H3F3A K27M Mutations Drive a Repressive Transcriptome by Modulating Chromatin Accessibility, Independent of H3K27me3 in Diffuse Midline Glioma [RNA-seq] 36 samples shares h3k27me3, chromatin
- GSE307922 Destabilization of the noncanonical PRC1.1 complex via USP7 inhibition induces neuronal differentiation in neuroblastoma [RNA-Seq] 18 samples shares prc1, canonical
- GSE294325 Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin 18 samples shares h3k27me3, chromatin
- GSE301505 Mutual Antagonism Between PRC1 Condensates and SWI/SNF in Chromatin Regulation 210 samples shares prc1, chromatin
- GSE280618 H3K27me3 chromatin heterogeneity reveals variable cell responses to estrogen and endocrine treatment 34 samples shares h3k27me3, chromatin
- GSE280617 H3K27me3 chromatin heterogeneity reveals variable cell responses to estrogen and endocrine treatment [RNA-seq] 18 samples shares h3k27me3, chromatin
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.