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Optimised, frameshift-driven design for single-cell CRISPR perturbation screens

GSE206490 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/12/31 Platform GPL24676
Summary
CRISPR-Cas9 has been widely used to functionally interrogate multiple aspects of cellular physiology and pathophysiology from single gene studies to genome-wide screens. Proper design of highly efficient guide RNAs directing the CRISPR genome editing process is critical for success in these types of experiments. Here, we present a pipeline for designing highly efficient loss-of-function guide RNA (gRNA) libraries with improved rates of knock-out efficiency compared to previous guide RNA library designs. We provide pre-computed and triaged gRNAs from our pipeline for all human and mouse transcripts through a fully searchable online portal as a resource to the community.
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Direct links to NCBI, no account and no request form: the whole study as GSE206490_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA851065 and SRA study SRP382724. Searching any of these in the dataset finder brings you back here.

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