Changes in gene expression in L-02 cells after treatment with PBAT material

We report the application of sequencing technology based on the reference transcriptome in L-02 cells. After using Cutadapt to filter out unqualified sequences (sequencing junctions, low quality sequences, etc.) from the raw data to obtain valid data (Clean Data), a reference genome alignment was performed using Hisat2. Based on the Hisat2 alignment results, transcripts were reconstructed using Stringtie and the expression levels of all genes in each sample were calculated. We found multiple down- and up-regulated genes in L-02 cells of the PBATc treatment group. Cluster heat maps were used to demonstrate differential gene expression patterns, and GO and KEGG pathway enrichment analyses were performed for differentially expressed genes. The results of GO functional annotation analysis showed that the largest number of differential genes were localized in biological processes, mainly including signal transduction, regulation of transcription and DNA-templated. In terms of cellular components, differential genes were mainly concentrated in In terms of cellular components, the differential genes are mainly in membrane, cytoplasm and nucleus, and the common Molecular Functions are protein binding, metal ion binding and DNA binding. The results of KEGG pathway analysis showed that there were differential genes in AMPK and mTOR pathways. Among them, AMPK, mTOR, SIRT1 and PGC-1α protein expression levels were down-regulated. Based on the above results, we examined each pathway protein. This study provides a strong reference for detecting the mechanism of cell damage after PBAT material treatment study.