← BioTransfer GEO Dataset Finder
GEO series

ChIP-seq analysis done for MBD3 before and after SP140 knockout in Ramos cells

GSE208134 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2024/11/21 Platform GPL21697
Summary
Sp140 is a lymphocytic-restricted protein, an epigenetic reader working as a corepressor of genes implicated in inflammation and orchestrating macrophage transcriptional programs to maintain cellular identity. Reduced Sp140 expression is associated both to autoimmune diseases and blood cancer. However, the molecular mechanisms that link Sp140 altered protein levels to detrimental effects on the immune response and cellular growth, as well as the interactors through which Sp140 promotes gene silencing, remain elusive. In this work, we have applied a multi-omics approach (i.e. interactomics, ChIP-seq and proteomics) in two Burkitt lymphoma cell lines to identify both interactors and target genes of endogenous Sp140. We found that Sp140 interacts with the PRC2 and NuRD complexes and we demonstrated that Sp140 interaction with PRC2 is functional and directs H3K27me3 deposition on a set of target genes related to cellular growth and leukaemia progression.
Published in
SP140 represses specific loci by recruiting polycomb repressive complex 2 and NuRD complex
Tamburri S, Zucchelli C, Matafora V et al. · Nucleic acids research 2025 · PMID 39718989 · doi:10.1093/nar/gkae1215
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE208134_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA858445 and SRA study SRP386270. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all human ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.