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Desciption of the transcriptome profiles of mouse heart innervating DRG and Nodose Ganglia-derived sensory neurons

GSE211622 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/08/19 Platform GPL19057
Summary
To obtain the mRNA expression profiles of vagal and spinal cardiac sensory neurons, mouse Dorsal Root Ganglia (DRG) and Nodose Ganglia (NG) neurons innervating the heart were labelled by retrograde Di-8-ANEPPQ dye which was injected to the heart. Then bilateral NGs and DRGs between C-T segments were dissected and Di-8-ANEPPQ-labelled DRG and NG neurons were FACS sorted. Finally, Di-8-ANEPPQ+ DRG and NG neurons were subjected to total RNA extraction and library preparation protocols for RNA-seq experiment. For control samples, total NG and DRG (C-T) neurons were dissected from the wt mice and same RNA extraction and library preparation protocols were applied. Finally, mRNA profiles of heart-spesific DRG and NG neurons were compared to those of total DRG and NG populations, respectively.
Published in
Heart-Specific Spinal and Vagal Afferents: Transcriptomic Signatures and Optogenetically Modulated Functional Coupling With Cardiomyocytes
Akgul Caglar T, Kazci YE, Durdu ZB et al. · Comprehensive Physiology 2026 · PMID 42381253 · doi:10.1002/cph4.70203
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Also filed as BioProject PRJNA871220 and SRA study SRP393115. Searching any of these in the dataset finder brings you back here.

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