← BioTransfer GEO Dataset Finder
GEO series

Transcriptomic and Metabolomic Analyses Reveal Distinctive Metabolic Characteristics of Superficial and Deep Subcutaneous Adipose Tissues

GSE216803 Homo sapiens (human) RNA-seq (gene expression) 12 samples Submitted 2024/10/28 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 12 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
12 samples: 6 × Superficial Sample vs 6 × Deep SampleGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
Background: The purpose of the study was to find out the molecular and metabolic characteristics of human superficial (SSAT) and deep subcutaneous adipose tissue (DSAT) by performing transcriptomics and metabolomics analysis Methods: We conducted RNA-sequencing and metabolome analysis of SSAT and DSAT in a 48-year-old female patient with a BMI of 27.6 kg/m2.
Read the full summary ↓
Six samples for each group were collected during a deep inferior epigastric perforator flap breast reconstruction. The calculated transcripts per million values were processed for unsupervised hierarchically clustered heat map generation, and Gene set enrichment analysis. For metabolomics analysis, the samples were analyzed in two modes for cationic and anionic metabolites by Capillary Electrophoresis Time-of-Flight Mass Spectrometry (CE-TOFMS). The metabolome was processed for principal component analysis (PCA) and heat map generation. For indirect metabolic flux analyses, primary adipocytes obtained from SSAT and DSAT were analyzed with Seahorse® extracellular flux analyzer. Results: PCA and heat map data revealed global differences in the transcriptome and metabolome of SSAT and DSAT. The top up-regulated gene sets in the SSAT were related to adipogenesis (FDR q < 0.0001), oxidative phosphorylation (FDR q < 0.0001), fatty acid metabolism (FDR q < 0.0001) and glycolysis (FDR q = 0.001), while inflammatory response (FDR q < 0.05) was the top up-regulated gene set in DSAT. Consequently, the metabolites related to glycolysis were abundant in SSAT, while the metabolites related to fatty acid metabolism and oxidative phosphorylation were abundant in DSAT. In cellular flux analysis, SSAT showed higher level of glycolysis and spare oxidative phosphorylation capacities. Conclusion: The global transcriptome and metabolome difference suggest that human superficial and deep subcutaneous adipose tissue are metabolically distinguishable subcompartments.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE216803_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA895419 and SRA study SRP405092. Searching any of these in the dataset finder brings you back here.

Study design
6 × Superficial Sample vs 6 × Deep Sample

Supports a between-group comparison across 12 samples.

How we read this: 2 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.