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Integrated transcriptomics of human blood vessels defines the spatially regulated niche for early mesenchymal progenitors

GSE217216 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/05/29 Platform GPL18573
Summary
Blood vessel walls are organized into concentric cellular layers, in which the outer layer, or tunica adventitia, represents the major niche for mesenchymal progenitor cells. By profiling large vessels (arteries and veins) using spatial transcriptomics, we found a hierarchy of stem cells based on spatial organization. In addition, we performed scRNaseq on CD34+ adventitial cells and integrated to out spatial transcriptomics where we identified the novel stem cell marker CD201 in the outer layer. This segregation was regulated by the WNT and TGFβ signaling pathways, which was confirmed by total RNAseq of CD201Low/High subsets of adventitial cells. To investigate the transcriptomic differences between the novel subset of of adventitial cells (CD34+,CD31-,CD45-) based on CD201 expression, FACTS isolated adventitial cells with low /high CD201 expression were sequenced.
Published in
Integrated transcriptomics of human blood vessels defines a spatially controlled niche for early mesenchymal progenitor cells
Wang Y, Thottappillil N, Gomez-Salazar M et al. · Developmental cell 2024 · PMID 39025061 · doi:10.1016/j.devcel.2024.06.015
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Also filed as BioProject PRJNA897919 and SRA study SRP406133. Searching any of these in the dataset finder brings you back here.

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