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N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations

GSE217243 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2024/05/12 Platform GPL18573
Summary
To investigate the effect of RPB1 CTD length on transcription dynamics, six mutant U2OS cell lines expressing tagged versions of α-amanitin resistant RPB1 with CTDs of varying lengths (Dendra2-RPB1-25R (D25), Dendra2-RPB1-52R (D52), Dendra2-RPB1-70R (D70), HaloTag-RPB1-25R (H25), HaloTag-RPB1-52R (H52), and HaloTag-RPB1-70R (H70)) were grown in α-amanitin to degrade endogenous RPB1, then their RNA was sequenced at single-cell resolution using a droplet-based system.
Published in
N-terminal tagging of RNA Polymerase II shapes transcriptomes more than C-terminal alterations
Callan-Sidat A, Zewdu E, Cavallaro M et al. · iScience 2024 · PMID 38799575 · doi:10.1016/j.isci.2024.109914
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Also filed as BioProject PRJNA898259 and SRA study SRP406264. Searching any of these in the dataset finder brings you back here.

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