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Effect of ablation of PRDM1 on gene expression in human regulatory T cells

GSE221348 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2025/07/23 Platform GPL24676
Summary
To gain an insight into the contribution of BLIMP-1 in human Tregs, we ablated PRDM1 in ex-vivo expaned human Tregs by Crispr/Cas9. Tregs transfected with scramble gRNAs were used as controls. 7 days after transfection, Tregs were rested overnight in media, and were re-stimulated through the TCR and IL2R for 16 hr prior to isolation of RNA for genome-wide RNA-seq. Gene expression profiling analysis was performed using data obtained from RNA-seq of four replicates of PRDM1KO and control cells.
Published in
BLIMP-1 and CEACAM1 cooperatively regulate human Treg homeostasis and function to control xenogeneic GVHD
Ding Y, Yu A, Vujanac M et al. · JCI insight 2025 · PMID 40773294 · doi:10.1172/jci.insight.183676
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Also filed as BioProject PRJNA913935 and SRA study SRP413918. Searching any of these in the dataset finder brings you back here.

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