Next Generation Sequencing facilitates to identify the differential expression genes of liver tissue from HBVN donors, HBVP donors, LF donors and transplant donors.

We obtained liver tissues from HBVN donors, HBVP donors, LF donors and transplant donors to rperforme next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with Illumina HiSeq 2000 and sequence analysis was determined using the Illumina data analysis pipeline. Based on the comparison results，we performed the GO and KEGG pathway enrichment analysis. That might suggest the different status of HBV-infected liver from liver surgery(AST<40) and liver transplantation(AST>40).