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Epigenetic changes from ATAC-seq of intestine stem cells from mice with graft-versus-host disease

GSE223814 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 7 samples Submitted 2024/09/11 Platform GPL24247
Summary
Intestinal stem cells (ISC) encounter inflammatory insults in immune mediated gastro-intestinal (GI) diseases. It remains unknown whether, and how, they adapt, and if the adaptation leaves scars on the ISCs that affects their subsequent regeneration capacity. We investigated the consequences of inflammation on Lgr5+ISCs in well-defined clinically relevant models of gastro-intestinal acute graft-versus-host disease (GI GVHD). Utilizing single cell transcriptomics, organoid, metabolic, epigenomic and in vivo models we found that Lgr5+ISCs undergo metabolic changes that lead to accumulation of succinate, which reprograms its epigenome. We performed transposase-accessible chromatin sequencing (ATAC-seq) in sorted Lgr5+ISCs harvested from the BALB/c→B6-GFP-Lgr5 model of GVHD.
Published in
Inflammation-induced epigenetic imprinting regulates intestinal stem cells
Zhao D, Ravikumar V, Leach TJ et al. · Cell stem cell 2024 · PMID 39232559 · doi:10.1016/j.stem.2024.08.006
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Also filed as BioProject PRJNA928449 and SRA study SRP419299. Searching any of these in the dataset finder brings you back here.

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