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Knockout of the B1 subunit of the GA-binding protein (GABP) [ChIP-seq]

GSE224164 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/06/11 Platform GPL24676
Summary
We knocked out different exons corresponding to different sections of GABP, a transcription factor known to bind to the telomerase reverse transcriptase (TERT) promoter with the G228 mutation. We investigated the effects of the knockouts in the regulation of TERT expression and other subunits of GABP. GABP genomic binding sites were determined through chromatin immunoprecipitation sequencing (ChIP-seq), and gene expression was determined through total RNA sequencing (RNA-seq). Combining data pertaining to GABP binding sites and gene expression provided insight into the molecular mechanisms of maintaining the length of telomeres in cancers.
Published in
The immortality mechanism of TERT promoter mutant cancers is self-reinforcing and reversible
Stevers NO, Kim S, Yuan JB et al. · Molecular cell 2025 · PMID 40513567 · doi:10.1016/j.molcel.2025.05.026
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Also filed as BioProject PRJNA929997 and SRA study SRP420312. Searching any of these in the dataset finder brings you back here.

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