← BioTransfer GEO Dataset Finder
GEO series

The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells [RNA-seq]

GSE225010 Homo sapiens Expression profiling by high throughput sequencing 8 samples Submitted 2026/03/30 Platform GPL16791
Summary
Various cellular stresses lead to an inhibition of steady-state translation driven by the cytoplasmic cap-binding protein eIF4E via the integrated stress response (ISR) in order to mitigate stress-induced damage. A caveat is that essential proteins for cell survival or maintenance should be expressed even under ISR-activated conditions. Here, we find that when global translation is compromised due to eIF2α phosphorylation during ISR, eIF2A takes over the function of eIF2α for Met-tRNAi delivery. Notably, preferential association between eIF2A and cellular factors involved in the pioneer round of translation driven by nuclear cap-binding protein complex (CBP20/80) allows the pioneer translation to occur during ISR. We also observe that ISR is activated in growing human embryonic stem cell (hESC) colonies. Under these naturally arising ISR conditions, eIF2A-mediated pioneer translation is active and safeguards self-renewal and differentiation of hESCs. Collectively, we unravel the molecular mechanism underlying translation that secures protein synthesis under cellular stress.
Published in
The nuclear cap-binding complex safeguards stress-resistant protein synthesis and proliferation of stem cells
Oh S, Chang J, Jo H et al. · Science advances 2026 · PMID 42054446 · doi:10.1126/sciadv.aea6630
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE225010_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA934591 and SRA study SRP422511. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 8 more — browse all 8 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.