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Gene expression profiling study to identify distinct subtypes in Peritoneal metastasis of colorectal cancer

GSE225182 Homo sapiens (human) RNA-seq (gene expression) 33 samples Submitted 2024/06/05 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 33 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Topic
Colorectal cancerThis series is part of our curated briefing on it.
Samples
33 samples: 33 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
CILP2 is a potential biomarker for the prediction and therapeutic target of peritoneal metastases in colorectal cancerScientific reports · 2024
Summary (written by the submitters)
The objective of this study is to assess gene expression changes and identify gene sets associated with metastasis into peritoneum in colorectal cancer (CRC) patients. We performed RNA-seq based transcriptome profiling using primary tumor samples obtained from 19 CRC patients with peritoneum metastasis. By a number of statistical tests, a total of 1,422 genes were statistically significant, indicating a gene set significantly associated with metastasis into peritoneum in CRC.
Read the full summary ↓
To select the genes showing strong gene expression intensities and critically validate the differences between CRCs with metastasis and without metasis, we applied more rigorous cut-off for gene selection, revealing candidates less than 20 genes. These genes were applied to the downstream experimental assays.
Published in
CILP2 is a potential biomarker for the prediction and therapeutic target of peritoneal metastases in colorectal cancer
Ha YJ, Park SH, Tak KH et al. · Scientific reports 2024 · PMID 38816545 · doi:10.1038/s41598-024-63366-4
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE225182_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA934310 and SRA study SRP422265. Searching any of these in the dataset finder brings you back here.

Study design
33 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 33 sample titles: 33 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
More Colorectal cancer datasets

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.