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Integrated single-cell analysis defines the epigenetic basis of castration-resistant prostate luminal cells (scATAC-seq)

GSE227107 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2024/06/14 Platform GPL24247
Summary
Understanding prostate response to castration and androgen receptor signaling inhibitors (ARSI) is critical to improving long-term prostate cancer (PCa) patient survival. Here we use a multi-omics approach on 229,794 single cells to create a mouse single-cell reference atlas better suited to interpreting mouse prostate biology and castration response. Our reference atlas refines single-cell annotations and provides chromatin context, which, when coupled with mouse lineage tracing demonstrates that the castration-resistant luminal cells are distinct from the pre-existent urethra-proximal stem/progenitor cells. Molecular pathway analysis and therapeutic studies further implicate JUN/FOS, WNT/b-Catenin, FOXQ1, NFkB, and JAK/STAT pathways as the major drivers of castration-resistant luminal populations with high relevance to human PCa. Importantly, we demonstrate the utility of our datasets, which can be explored through an interactive portal (https://visportal.roswellpark.org/data/tang/), to aid in developing novel combination treatments with ARSI for advanced PCa patients.
Published in
Integrated single-cell analysis defines the epigenetic basis of castration-resistant prostate luminal cells
Kirk JS, Wang J, Long M et al. · Cell stem cell 2024 · PMID 38878775 · doi:10.1016/j.stem.2024.05.008
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Direct links to NCBI, no account and no request form: the whole study as GSE227107_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA943238 and SRA study SRP426636. Searching any of these in the dataset finder brings you back here.

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