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Single-cell Multi-omics Analysis of Human Testicular Germ Cell Tumor Reveals its Molecular Features and Microenvironment

GSE228501 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2024/03/23 Platform GPL24676
Summary
Seminoma is the most common malignant solid tumor in 14 to 44 year-old men. However, its molecular features and tumor microenvironment (TME) is largely unexplored. Here, we performed a series of studies via genomics profiling (single cell multi-omics and spatial transcriptomics) and functional examination using seminoma samples and a seminoma cell line. We identified key gene expression programs shared between seminoma and primordial germ cells, and further characterized the functions of TFAP2C in promoting tumor invasion and migration. We also identified 15 immune cell subtypes in TME, and found that subtypes with exhaustion features were located closer to the tumor region through combined spatial transcriptome analysis. Furthermore, we identified key pathways and genes that may facilitate seminoma breaking through the seminiferous tubules. These findings advanced our knowledge of seminoma tumorigenesis and produced a multi-omics atlas of in situ human seminoma microenvironment, which could help discover potential therapy targets for seminoma.
Published in
Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment
Lu X, Luo Y, Nie X et al. · Nature communications 2023 · PMID 38123589 · doi:10.1038/s41467-023-44305-9
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Direct links to NCBI, no account and no request form: the whole study as GSE228501_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA950062 and SRA study SRP430003. Searching any of these in the dataset finder brings you back here.

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