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Alzheimer’s disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAs [BV-2 cells]

GSE229416 Mus musculus (mouse) RNA-seq (gene expression) 32 samples Submitted 2024/03/20 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
32 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Alzheimer's disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAsAlzheimer's & dementia : the journal of the Alzheimer's Association · 2024
Summary (written by the submitters)
To detect miRNA targets in glioblastoma cell line (BV-2) we stimulate the cells with LPS 10ng/ml or vehicle and then transfect them with miRNA mimics or scrable control We performed differentially expression analysis of RNA-seq BV-2 cells vehicle or LPS stimulated overexpressing miRNA mimic or scramble mimic
Published in
Alzheimer's disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAs
Scoyni F, Giudice L, Väänänen MA et al. · Alzheimer's & dementia : the journal of the Alzheimer's Association 2024 · PMID 37828821 · doi:10.1002/alz.13502
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE229416_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA954387 and SRA study SRP432023. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 32 more — browse all 32 samples with per-sample file links →

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