← BioTransfer GEO Dataset Finder
GEO series

Embryonic macrophages support endocrine commitment during human pancreatic differentiation

GSE230403 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/10/14 Platform GPL24676
Summary
Organogenesis is a complex process that relies on a dynamic interplay between extrinsic factors originating from the microenvironment and tissue-specific intrinsic factors. For pancreatic endocrine cells, the local niche consists of acinar and ductal cells as well as neuronal, immune, endothelial, and stromal cells. Hematopoietic cells have been detected in human pancreas as early as 6 post-conception weeks, but whether they play a role during human endocrinogenesis remains unknown. To investigate this, we performed single nuclei RNA sequencing of the second trimester human pancreas and identified a wide range of hematopoietic cells, including two distinct subsets of tissue-resident macrophages. Leveraging this discovery, we developed a co-culture system of human embryonic stem cell-derived endocrine-macrophage organoids to model their interaction in vitro. Here, we show that macrophages support the differentiation and viability of endocrine cells in vitro and enhance tissue engraftment, highlighting their potential role in tissue engineering strategies for diabetes.
Published in
Embryonic macrophages support endocrine commitment during human pancreatic differentiation
Migliorini A, Ge S, Atkins MH et al. · Cell stem cell 2024 · PMID 39406230 · doi:10.1016/j.stem.2024.09.011
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE230403_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA961074 and SRA study SRP434133. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.