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In vivo clonal tracking of human skeletal stem cells with single cell RNA-sequencing readout

GSE233188 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/02/25 Platform GPL15520Platform GPL24676
Summary
10X Genomics single cell RNA-sequencing of freshly isolated human skeletal stem cells from the growth plate or periosteum of 17 weeks gestational age fetal bone tissue were collected. The goal of this work was to trace single cell dynamics of human skeletal stem cells in an in vivo setting. Single barcoded human skeletal stem cells were tracked in the graft tissue they formed after xenotransplanation into mice. Single cell analysis assessed tissue composition and was overlayed with barcode readouts to find contributions of individual clones. Differences in stem cell properties and lineage dynamics were assessed between growth plate and periosteal human skeletal stem cells.
Published in
Human skeletal development and regeneration are shaped by functional diversity of stem cells across skeletal sites
Ambrosi TH, Taheri S, Chen K et al. · Cell stem cell 2025 · PMID 40118065 · doi:10.1016/j.stem.2025.02.013
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Also filed as BioProject PRJNA975197 and SRA study SRP439086. Searching any of these in the dataset finder brings you back here.

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