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Human spinal cord organoids revealed cell intercalation as a conserved morphogenic mechanism for lumem formation

GSE233686 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/06/30 Platform GPL28038
Summary
Neural tube defects (NTDs) are severe anatomical malformations of the nervous system that occur during embryonic development when the neural tube (NT) fails to form correctly. To gain insights into the biological basis of NTDs, we generated human NT organoids with defined anterior and posterior identities. We achieved this by guiding human pluripotent stem cells towards the neural lineage, resulting in the self-assembly of polarized cells around a single lumen, exhibiting subcellular architecture and dynamics similar to those of the embryonic NT. Through transcriptome profiling, we identified that the posterior organoids possessed a spinal cord identity. To better replicate the in vivo morphogenesis of the secondary NT, we developed a modified culture condition, which revealed that lumen resolution in the spinal cord organoids involves cell intercalation and is dependent on the activity of Yes-associated protein (YAP). We believe that these findings will contribute to our understanding of caudal skin-covered NTDs, which are among the most common birth defects observed in humans.
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Also filed as BioProject PRJNA977600 and SRA study SRP440123. Searching any of these in the dataset finder brings you back here.

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