Bulk RNA-sequencing from SVBP or TTL gene knock-out and WT engineered heart tissues

For this study, we used commercially available hiPSCs (Allen Institute, AICS-0031) and created knock-out lines for small vasohibin binding protein (SVBP) and tubulin tyrosine ligase (TTL), respectively. These hiPSCs were then differentiated into cardiomyocytes using a monolayer protocol and then further used for generation of engineered heart tissues (EHTs) to more closely model human physiology. EHTs were either cultured on control silicone posts or stiffer silicone posts to achieve enhanced afterload.