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The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation in vivo

GSE234448 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2024/06/25 Platform GPL24247
Summary
Highly dynamic chromosome remodeling and extensive nuclear compartmentalization occur during mammalian meiotic prophase I. We report here that mouse spermatocytes lacking MAPS, the male pachynema-specific protein, exhibit multiple pachytene defects, including disordered chromatin accessibility, interrupted nucleosome composition, and globally altered transcription, accompanied by disturbed nucleus phase formation. Adult Maps−/− pachytene spermatocytes lack sex body formation and exhibit improper autosomal chromatin condensation, which can be attributed to the feature of MAPS to mediate phase separation through its unique intrinsically disordered regions (IDRs), amino acids 2-9. MAPS protein with deletion of IDR failed to form a distinguishable phase in vitro and in cultured cells, and remarkably, MAPS IDR-deleted male mice suffer from pachytene arrest and sterility, with a typical absence of sex body and less condensed autosomal chromosomes, similar to Maps−/− mice. Thus, the nucleus phase separation mediated by MAPS may be essential for male pachynema progression in mice.
Published in
The male pachynema-specific protein MAPS drives phase separation in vitro and regulates sex body formation and chromatin behaviors in vivo
Lin Z, Li D, Zheng J et al. · Cell reports 2024 · PMID 38175751 · doi:10.1016/j.celrep.2023.113651
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Also filed as BioProject PRJNA981461 and SRA study SRP441804. Searching any of these in the dataset finder brings you back here.

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