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Long Non-Coding RNA MEG3 Promotes Angiogenesis by directly regulating integrin signaling impairing endothelial cell function [ChIP-seq]

GSE234543 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2024/04/12 Platform GPL20301
Summary
We immunoprecipitated EZH2, together with associated chromatin isolated from the HUVECs (2 x 10^6) that were transfected with MEG3 GapmeRs (10 nM, 48 h) or a scrambled control GapmeRs (Ctr). For transfection, we used 10 nM scrambled LNA (locked nucleic acids) GapmeR control (Cat. No. 339515) or phosphorothioate antisense standard GapmeRs MEG3-lncRNA (Cat No. 339511, Qiagen). On beads crosslinked chromatin complexes were reversed, and DNA purified using QIAquick® PCR Purification Kit and quantified by Qubit HS assay (Q33230)
Published in
Control of endothelial cell function and arteriogenesis by MEG3:EZH2 epigenetic regulation of integrin expression
Dunn-Davies H, Dudnakova T, Nogara A et al. · Molecular therapy. Nucleic acids 2024 · PMID 38617973 · doi:10.1016/j.omtn.2024.102173
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Also filed as BioProject PRJNA982001 and SRA study SRP441972. Searching any of these in the dataset finder brings you back here.

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