An essential role for the ultra-conserved poison exon of Tra2b in fertility in mice
RNA-seq (gene expression) · mouse · 11 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 11 samples: 9 distinct titles, 1 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- An ultra-conserved poison exon in the Tra2b gene encoding a splicing activator is essential for male fertility and meiotic cell divisionThe EMBO journal · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE235085_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA984307 and SRA study SRP444260. Searching any of these in the dataset finder brings you back here.
How we read this: Read from 11 sample titles: 9 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7494605 Tra2beta iCLIP 1
- GSM7494606 Tra2beta iCLIP 2
- GSM7494607 Tra2beta iCLIP 3
- GSM7494608 P12 neonatal wild type testis, rep A
- GSM7494609 P12 neonatal wild type testis, rep B
- GSM7494610 P12 neonatal wild type testis, rep C
- GSM7494611 P12 neonatal wild type testis, rep D
- GSM7494612 P12 neonatal Tra2b-cPEko, rep A
- GSM7494613 P12 neonatal Tra2b-cPEko, rep B
- GSM7494614 P12 neonatal Tra2b-cPEko, rep C
- GSM7494615 P12 neonatal Tra2b-cPEko, rep D
- GSE267603 Tra2b's Ultraconserved Poison Exon relays TCR stimulation to shape T cell fate [Tra2bKO] 18 samples shares tra2b, poison, exon
- GSE267608 Tra2b's Ultraconserved Poison Exon relays TCR stimulation to shape T cell fate [mTorSeq] 11 samples shares tra2b, poison, exon
- GSE279216 ISWI chromatin remodeler SMARCA5 is essential for meiotic gene expression and male fertility in mammals 10 samples shares essential, fertility
- GSE215762 Mettl3-dependent m6A modification is essential for effector differentiation and memory formation of CD8+ T cells 41 samples shares essential
- GSE318098 DDX3X-Mediated Translation of Structured Cardiac mRNAs is Essential for Female Heart Development [Ribo-Seq] 12 samples shares essential
- GSE267538 Nr5a2 is dispensable for ZGA but essential for morula development 537 samples shares essential
- GSE279446 Derepression of a single microRNA target causes female infertility in mice [RNA-Seq] 69 samples shares fertility
- GSE223673 Nr5a2 is dispensable for ZGA but essential for morula development [RNA-seq] 514 samples shares essential
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.