GEO series

Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm

GSE235161 Homo sapiens (human) RNA-seq (gene expression) 42 samples Submitted 2024/11/19 Platform GPL23227

At a glance

RNA-seq (gene expression) · human · 42 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Homo sapiens (human)
Samples: 42 samples: 42 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Mitochondrial NAD(+) deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysmNature cardiovascular research · 2025

Summary (written by the submitters)

Thoracic and abdominal aortic aneurysm poses a substantial mortality risk in adults, yet many of its underlying factors remain unidentified. Here, we identify mitochondrial nicotinamide adenine dinucleotide (NAD)⁺ deficiency as a causal factor for the development of aortic aneurysm. Multiomics analysis of 150 surgical aortic specimens indicated impaired NAD+ salvage and mitochondrial transport in human thoracic aortic aneurysm, with expression of the NAD+ transporter SLC25A51 inversely correlating with disease severity and postoperative progression.

Read the full summary ↓

Genome-wide gene-based association analysis further linked low SLC25A51 expression to risk of aortic aneurysm and dissection. In mouse models, smooth muscle-specific knockout of Nampt, Nmnat1, Nmnat3, Slc25a51, Nadk2 and Aldh18a1, genes involved in NAD+ salvage and transport, induced aortic aneurysm, with Slc25a51 deletion producing the most severe effects. Using these models, we suggest a mechanism that may explain the disease pathogenesis: the production of type III procollagen during aortic medial matrix turnover imposes a high demand for proline, an essential amino acid component of collagen. Deficiency in the mitochondrial NAD⁺ pool, regulated by NAD⁺ salvage and transport, hinders proline biosynthesis in mitochondria, contributing to thoracic and abdominal aortic aneurysm.

Published in

Mitochondrial NAD(+) deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm

Zhang J, Tang Y, Zhang S et al. · Nature cardiovascular research 2025 · PMID 39843801 · doi:10.1038/s44161-024-00606-w

This dataset

Open GSE235161 in finder → Find more human RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE235161_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA984692 and SRA study SRP444429. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 39843801) ↗

Study design

42 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 42 sample titles: 42 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM7496121 TAA_4 [B10]
GSM7496122 TAA_16 [B35]
GSM7496123 TAA_21 [B49]
GSM7496124 TAA_29 [B62]
GSM7496125 TAA_30 [B64]
GSM7496126 TAA_36 [B74]
GSM7496127 TAA_39 [B78]
GSM7496128 TAA_40 [B81]
GSM7496129 TAA_42 [B86]
GSM7496130 TAA_45 [B90]
GSM7496131 TAA_47 [B94]
GSM7496132 TAA_51 [D2]
GSM7496133 TAA_56 [D9]
GSM7496134 TAA_62 [D15]
GSM7496135 TAA_64 [D17]
GSM7496136 TAA_65 [D18]
GSM7496137 TAA_67 [D7]
GSM7496138 TAA_68 [M3]
GSM7496139 TAA_69 [M4]
GSM7496140 TAA_70 [M5]
GSM7496141 TAA_71 [M6]
GSM7496142 TAA_73 [M8]
GSM7496143 TAA_74 [M9]
GSM7496144 TAA_75 [M10]
GSM7496145 TAA_78 [T14]
GSM7496146 TAA_88 [T28]
GSM7496147 TAA_103 [T55]
GSM7496148 TAA_104 [T56]
GSM7496149 TAA_105 [T59]
GSM7496150 TAA_111 [T68]
GSM7496151 TAA_112 [T69]
GSM7496152 Control_5 [N8]
GSM7496153 Control_10 [N14]
GSM7496154 Control_12 [N19]
GSM7496155 Control_14 [N21]
GSM7496156 Control_16 [N24]
GSM7496157 Control_18 [N27]
GSM7496158 Control_20 [N29]
GSM7496159 Control_22 [N31]
GSM7496160 Control_23 [N32]

+ 2 more — browse all 42 samples with per-sample file links →

Datasets on a similar topic

GSE318549 NAD-dependent redox control enables endothelial quiescence and vascular stabilization during angiogenesis 15 samples shares nad, vascular
GSE312780 Blood-flow restriction resistance training improves skeletal muscle mitochondrial oxidative capacity and cardiovascular risk factors in type 2 diabetes 34 samples shares mitochondrial, vascular
GSE343456 MCART1 (SLC25A51) deficiency exacerbates mitochondrial dysfunction during MPP+-induced complex I stress 24 samples shares mitochondrial, deficiency
GSE302114 Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production 18 samples shares mitochondrial, collagen
GSE228133 Deficiency of Smooth Muscle ADAR1 RNA Editing Exacerbates Vascular Remodeling and Pulmonary Hypertension [pul43_RNA-seq] 12 samples shares deficiency, vascular
GSE261892 Australian Chronic Allograft Dysfunction III 123 samples shares iii
GSE333557 RNA Polymerase III inhinition leads to changes in protein coding gene expression 54 samples shares iii
GSE309841 Integrative tissue analysis of men with prostate cancer VIII 42 samples shares iii

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.