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Compartment Specific Mechanical Loading of Bi-Layered Osteochondral Micro-Tissues in a Joint-on-Chip System suggests routes for Osteoarthritis pathogenesis investigation

GSE237786 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/09/17 Platform GPL24676
Summary
In this work we engineered an osteochondral unit (OCU) on-chip model where osteoarthritis (OA) phenotype is recapitulated through the application of hyper-physiological mechanical loading. After a functional and biological validation of the device, we aimed to better characterize the diversity of the human articular chondrocytes (hACs) population present in our in vitro model and identify subpopulations responsible for the pathological loading response. As a method, we applied single-cell RNA sequencing (scRNA-seq) to hACs from both on-chip single cultures and co-cultures with bone marrow derived mesenchymal stromal cells (bmMSCs), i.e. in osteochondral construct, and either exposed or not to cyclic mechanical loading.
Published in
An Organ-on-Chip Platform for Strain-Controlled, Tissue-Specific Compression of Cartilage and Mineralized Osteochondral Interface to Study Mechanical Overloading in Osteoarthritis
Mainardi A, Börsch A, Occhetta P et al. · Advanced healthcare materials 2025 · PMID 40556597 · doi:10.1002/adhm.202501588
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Also filed as BioProject PRJNA996606. Searching any of these in the dataset finder brings you back here.

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