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Histone H3 acetylation (pan-acetyl) of WT, Mpc2-deficient basal cells and acetate treated Mpc2-deficient basal cells

GSE240346 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 9 samples Submitted 2025/01/08 Platform GPL24247
Summary
To further access whether changes in histone acetylation are directly associated with cell differentiation, we performed CUT&Tag experiment on WT cells, Mpc2-deficient cells, and acetate treated Mpc2-deficient cells by using antibody against H3 pan-acetylation. Mpc2 mutant cells displayed a decrease in the peaks of histone H3 acetylation near transcription start sites compared to control cells, whereas this reduction could be partially reversed by acetate supplementation. Pathway analysis using differentially acetylated genes between control and Mpc2 mutant cells revealed alterations in differentiation-related signaling pathways, such as TGF-β and Notch. Indeed, Mpc2 mutant BCs showed insufficient H3 acetylation at the promoters of key differentiation related genes, such as Hes1 and HeyL, downstream transcriptional factor of Notch signaling, and the decrease could be partially reversed by acetate treatment.
Published in
Mitochondrial pyruvate carriers control airway basal progenitor cell function through glycolytic-epigenetic reprogramming
Li Y, He Y, Zheng Q et al. · Cell stem cell 2025 · PMID 39426380 · doi:10.1016/j.stem.2024.09.015
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Also filed as BioProject PRJNA1003413 and SRA study SRP454001. Searching any of these in the dataset finder brings you back here.

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