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The role of HNRNPU and the nuclear matrix in human pluripotent stem cells [ATAC]

GSE242341 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2024/11/05 Platform GPL24676
Summary
During early embryogenesis the fertilized zygote proceeds through an intricate developmental program, accompanyed by DNA and chromatin remodelling. However, the mechanisms governing this reconfiguring are poorly understood. Parts of the embryogenesis developmental program can be captured in vitro in the form of two types of human pluripotent stem cells (hPSC): naïve and primed hPSCs. Naïve hPSCs resemble the inner cell mass of the blastocyst, whilst primed hPSCs resemble the post-implantation embryo. The nucleus is made of DNA wrapped around chromatin, and the nuclear matrix, a proteinaceous gel that provides structure. Here, we show that disruptions in the nuclear matrix caused primed hPSCs to spontaneously convert to the naïve, earlier embryonic state.
Published in
BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation
Sun L, Fu X, Xiao Z et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 · PMID 39656858 · doi:10.1002/advs.202409160
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Also filed as BioProject PRJNA1013378 and SRA study SRP458923. Searching any of these in the dataset finder brings you back here.

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