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Evaluating TF-target gene interactions in the placenta using siRNA knockdowns

GSE245394 Homo sapiens Expression profiling by high throughput sequencing 70 samples 2024/07/03 GPL24676
Summary
Gene regulation is essential to placental function and fetal development. We built a genome-scale transcriptional regulatory network (TRN) of the human placenta using digital genomic footprinting and transcriptomic data. We integrated 475 transcriptomes and 12 DNase hypersensitivity datasets from placental samples to globally and quantitatively map transcription factor (TF)-target gene interactions. We performed siRNA knockdowns of 4 TFs and achieved concordance between the predicted gene targets in our TRN. This GEO dataset summarizes the results of the knockdowns for these 4 TFs: GCM1, CREB2L2, ESRRG, and GRHL2 compared to siRNA controls (SCR).
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