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Identification of differentially expressed tumour-related genes regulated by UHRF1 regulated DNA methylation [RNA-Seq]

GSE248620 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/05/09 Platform GPL24676
Summary
UHRF1 is an epigenetic regulator that plays critical roles in tumours. However, the DNA methylation alteration patterns driven by UHRF1 and the related differentially expressed tumour-related genes remain unclear. In this study, a UHRF1-shRNA MCF-7 cell line was constructed, and whole-genome bisulfite sequencing (WGBS) and RNA sequencing were performed. The DNA methylation alteration landscape was elucidated, and DNA methylation-altered regions (DMRs) were found to be distributed in both gene bodies and adjacent regions. The DMRs were annotated and categorized into 488 hypermethylated/1696 hypomethylated promoters and 1149 hypermethylated/5501 hypomethylated gene bodies. Through an integrated analysis with the RNA sequencing data, 217 upregulated methylated genes and 288 downregulated methylated genes were identified, and these genes were primarily enriched in nervous system development and cancer signalling pathways.
Published in
Identification of differentially expressed tumour-related genes regulated by UHRF1-driven DNA methylation
Dong Q, Gong C, Jiang Q et al. · Scientific reports 2024 · PMID 39112494 · doi:10.1038/s41598-024-69110-2
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Also filed as BioProject PRJNA1045263 and SRA study SRP474230. Searching any of these in the dataset finder brings you back here.

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