← BioTransfer GEO Dataset Finder
GEO series

EIF3D Safeguards the Homeostasis of Key Signaling Pathways in Human Primed Pluripotency [Ribo-seq]

GSE249191 Homo sapiens Expression profiling by high throughput sequencing; Other 6 samples Submitted 2025/04/10 Platform GPL30173
Summary
Pluripotent stem cell identities such as differentiation and infinite proliferation have long been decoded in the frameworks of transcription factor networks, epigenomes, and signal transduction, yet unclear and fragmented. However, directing attention toward translation regulation, the bridge between these events promises to provide new insights into previously unexplained mechanisms. Functional screening led to the discovery that EIF3D maintains primed pluripotency via selective translation regulation. The loss of EIF3D unbalanced the pluripotency-associated signaling pathways, disrupting primed pluripotency. Furthermore, we found that EIF3D safeguards robust proliferation by managing the translation of multiple p53 regulators that maintain low p53 activity in the undifferentiated state. Therefore, this study provides a paradigm for selective translation regulation that defines the primed pluripotent stem cell identity.
Published in
EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency
Okubo C, Nakamura M, Sato M et al. · Science advances 2025 · PMID 40203091 · doi:10.1126/sciadv.adq5484
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE249191_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1047636 and SRA study SRP475394. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 6 more — browse all 6 samples with per-sample file links →

Similar datasets

Search all human RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.