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Identification of a human hematopoietic stem cell subset that retains memory of inflammatory stress [Xenograft]

GSE249478 Homo sapiens Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2026/03/09 Platform GPL24676
Summary
Hematopoietic stem cells (HSCs) survive a lifetime of infection to sustain life-long blood production. Inflammation activates many blood cell types, driving aging and malignancy. To understand HSC adaptation to inflammation, we performed single cell multi-omics on human HSC in xenograft inflammation-recovery models. Two transcriptionally and epigenetically distinct HSC subsets expressing canonical HSC programs were identified. Only one showed sustained transcriptional and epigenetic changes after recovery from inflammatory treatments. This HSC inflammatory memory (HSC-iM) program is enriched in memory T cells and HSC from recovered COVID-19 patients. Importantly, HSC-iM accumulates with age and in clonal hematopoiesis. Overall, heritable molecular alterations in a subset of human HSCs, an adaptation to long-term inflammatory stress, may predispose to heightened age related risk of blood cancer and infection.
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Direct links to NCBI, no account and no request form: the whole study as GSE249478_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1049358. Searching any of these in the dataset finder brings you back here.

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