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Hijacking the transcriptional potential of the BAF complex for therapeutic purposes via induce proximity

GSE249747 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/12/04 Platform GPL24676
Summary
The BAF (Brg/Brahma-associated factors) complex, also referred to as the mammalian Switch/Sucrose-Nonfermentable (mSWI/SNF) chromatin remodeling complex, plays a pivotal role in epigenetically regulating diverse transcriptional programs. BAF’s chromatin remodeling activity, which enhances accessibility to transcriptional machinery, is critical for gene regulation. We hypothesize that redirecting endogenous BAF complexes to “turn on” therapeutically relevant genes offer an additional approach for disease treatment. Using chronic myelogenous leukemia K562 cells stably expressing the FIRE-dCas9 system (K562-FIRE) and rapamycin as a chemical inducer of proximity (CIP) via CRISPR/Cas9 and FKBP/FRB dimerization, we redirected endogenous BAF complexes to the promoter of fetal hemoglobin (HBG) using pooled small guide RNAs (sgRNAs) targeting the HBG1 promoter. RNA-seq analysis showed a statistically significant increase in HBG1 and HBG2 gene expression in rapamycin treated cells over the DMSO treated controls.
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Also filed as BioProject PRJNA1050280 and SRA study SRP476695. Searching any of these in the dataset finder brings you back here.

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