USP10-mediated PFKFB4 deubiquitination induces lung cancer radioresistance by activating the fumarate/Rad51 axis
RNA-seq (gene expression) · human · 9 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Topic
- Lung cancerThis series is part of our curated briefing on it.
- Samples
- 9 samples: 3 × H1299 cells, Scramble vs 3 × H1299 cells, SiPFKFB4-1 vs 3 × H1299 cells, SiPFKFB4-2Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE249850_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1051015 and SRA study SRP477273. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 9 samples.
How we read this: 3 replicated groups read from 9 sample titles; they account for 9 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7965962 H1299 cells, Scramble 1 H1299 cells, Scramble
- GSM7965963 H1299 cells, Scramble 2 H1299 cells, Scramble
- GSM7965964 H1299 cells, Scramble 3 H1299 cells, Scramble
- GSM7965965 H1299 cells, SiPFKFB4-1 1 H1299 cells, SiPFKFB4-1
- GSM7965966 H1299 cells, SiPFKFB4-1 2 H1299 cells, SiPFKFB4-1
- GSM7965967 H1299 cells, SiPFKFB4-1 3 H1299 cells, SiPFKFB4-1
- GSM7965968 H1299 cells, SiPFKFB4-2 1 H1299 cells, SiPFKFB4-2
- GSM7965969 H1299 cells, SiPFKFB4-2 2 H1299 cells, SiPFKFB4-2
- GSM7965970 H1299 cells, SiPFKFB4-2 3 H1299 cells, SiPFKFB4-2
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.