Isoleucyl-tRNA synthetase 1 novel mutation promotes deposition of pulmonary surfactant through stabilizing Cathepsin Z
RNA-seq (gene expression) · human · 14 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 14 samples: 6 × p28d-WT vs 6 × p28d-MutGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE250412_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1053925 and SRA study SRP478212. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 2 replicated groups read from 14 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM7976503 p28d-WT-1 p28d-WT
- GSM7976504 p28d-WT-3 p28d-WT
- GSM7976505 p28d-WT-4 p28d-WT
- GSM7976506 p28d-WT-5 p28d-WT
- GSM7976507 p28d-WT-6 p28d-WT
- GSM7976508 p28d-WT-7 p28d-WT
- GSM7976509 p28d-Mut-1 p28d-Mut
- GSM7976510 p28d-Mut-2 p28d-Mut
- GSM7976511 p28d-Mut-3 p28d-Mut
- GSM7976512 p28d-Mut-4 p28d-Mut
- GSM7976513 p28d-Mut-5 p28d-Mut
- GSM7976514 p28d-Mut-7 p28d-Mut
- GSM7978790 IARS1 mutation 1
- GSM7978791 IARS1 healthy control 1
- GSE245173 APE1 regulates the transcription of urea cycle genes through stabilizing DNA G-quadruplexes in lung adenocarcinoma 38 samples shares stabilizing
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.