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The role of HELLS in human induced pluripotent stem cells II

GSE250465 Homo sapiens Genome binding/occupancy profiling by high throughput sequencing 8 samples Submitted 2025/06/23 Platform GPL24676
Summary
interactions that require remodeling proteins like the Helicase, Lymphoid-specific (HELLS). Here, we generate HELLS and de novo DNA methyltransferase 3 A and B (DNMT3A/B) knockout hu-man pluripotent stem cells and assemble telomere-to-telomere maps of whole genome bisulfite sequencing data combined with ATAC-sequencing. Disrupting HELLS induces a global loss of DNA methylation that is distinct from the de novo DNMTs, in particular over peri/centromeric satellite repeats as defined in the telomere-to-telomere genome assembly. However, HELLS is dispensable for local enhancer remodeling and the potential to differentiate into the three germ layers. Taken together, these findings further clarify the genomic targets and role of HELLS in human cells.
Published in
HELLS is required for maintaining proper DNA modification at human satellite repeats
Guckelberger P, Haut L, Tornisiello R et al. · Genome biology 2025 · PMID 40676590 · doi:10.1186/s13059-025-03681-9
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Also filed as BioProject PRJNA1054110 and SRA study SRP478724. Searching any of these in the dataset finder brings you back here.

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