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Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei [RNA-seq]

GSE251678 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2024/08/28 Platform GPL24676Platform GPL20795
Summary
Brain organoids with nucleus-specific identities provide unique platforms for studying human brain development and diseases at a finer resolution. Despite its essential role in vital body functions, the medulla of the hindbrain has seen a lack of in vitro models, let alone models resembling specific medullary nuclei, including the crucial spinal trigeminal nucleus (SpV) that relays peripheral sensory signals to the thalamus. Here, we report a method to differentiate human pluripotent stem cells into region-specific brain organoids resembling the dorsal domain of the medullary hindbrain. Importantly, organoids specifically recapitulated the development of SpV derived from the dorsal medulla. We also developed an organoid system to create the trigeminothalamic projections between the SpV and the thalamus by fusing these organoids, namely human medullary SpV-like organoids (hmSpVOs), with organoids representing the thalamus (hThOs). Our study provides a platform for understanding SpV development, nucleus-based circuit organizations, and related disorders in the human brain.
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Also filed as BioProject PRJNA1055053 and SRA study SRP479321. Searching any of these in the dataset finder brings you back here.

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