Biphasic response of human iPSC-derived neural network activity following exposure to a sarin-surrogate nerve agent

In a concentration-response study, we have employed a human-relevant brain microphysiological system (bMPS), containing human-iPSC derived glutamatergic and GABAergic neurons co-cultured with primary human astrocytes, to monitor and detect the changes in the electrical activity of engineered human iPSC-derived neural networks to increasing concentrations of the sarin surrogate, 4-nitrophenylisopropyl methylphosphonate (NIMP). Transcriptomic analysis was conducted at 6-days post exposure to evaluate gene expression changes that could have contributed to how neurons communicated following NIMP exposure.